Goat milk as a non-invasive sample for confirmation of Mycobacterium avium subspecies paratuberculosis by IS900 PCR
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چکیده
منابع مشابه
Goat paratuberculosis in Chile: first isolation and confirmation of Mycobacterium avium subspecies paratuberculosis infection in a dairy goat.
In October 2004, 41 goats > 2 years old from a Saanen dairy goat herd located in Purranque County, 10th Region, Chile, were sampled and tested for paratuberculosis. While collecting samples it was observed that several goats were thin and emaciated. One goat was sufficiently debilitated to warrant humane euthanasia. This animal was brought to the Veterinary School at the Universidad Austral de ...
متن کاملDiagnostic Application of IS900 PCR Using Blood as a Source Sample for the Detection of Mycobacterium avium Subspecies Paratuberculosis in Early and Subclinical Cases of Caprine Paratuberculosis
Efficacy of IS900 blood PCR was evaluated for the presence of MAP infection. Serum, fecal, and blood samples of kids, young, and adult goats from farm and farmer's herds in Mathura district were also screened by ELISA, microscopy and culture. Of 111 goats (kids: 40, young: 14, adults: 57) screened, 77.5% were positive by blood PCR. Of 76 goats, 90.8% (kids: 87.5% and adults: 94.4%) were positiv...
متن کاملColostrum and milk as risk factors for infection with Mycobacterium avium subspecies paratuberculosis in dairy cattle.
Mycobacterium avium ssp. paratuberculosis (MAP) infections cause major losses to the dairy industry. Transmission of MAP occurs primarily via feces and in utero, but MAP can also be excreted in colostrum and milk. The objective of this study was to determine whether colostrum and milk fed to calves are important risk factors for infection with MAP. A questionnaire was sent to 1,050 farms partic...
متن کاملThe Mycobacterium avium Subspecies Paratuberculosis Dilemma
The purpose of this paper is to present and update the longitudinal perspective of the Mycobacterium avium subspecies paratuberculosis dilemma as initially stated in Infectious Diseases Incorporated’s 2012 White Paper. In both wild and domestic herbivores, Mycobacterium avium subspecies paratuberculosis (MAP) produces chronic granulomatous enteritis (Johne’s disease) that, if unchecked, is usua...
متن کاملGenomic Characterization of the Vaccinal Strain of Mycobacterium Avium Subspecies Paratuberculosis (MAP) 316F by MIRU-VNTR
Abstract Background and Objective: Paratuberculosis is a chronic granulomatous enteritis of ruminants caused by Mycobacterium avium subspecies paratuberculosis (MAP). this study aimed to characterize the genome of the MAP 316F strain. Methods: The MAP 316F strain was subjected to the PCR-F57 and PCR-IS900 experi...
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ژورنال
عنوان ژورنال: Journal of Advanced Veterinary and Animal Research
سال: 2014
ISSN: 2311-7710
DOI: 10.5455/javar.2014.a25